Premier
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 3 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 1 ELISA-positive clone
Pro
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
Plus
ProSci-Provided Antigen
Time: ~22-30 weeks
4-5 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 25 aa at >90% purity
- Conjugation to carrier protein
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
