Serum
Client-Provided Antigen
Time: 9 weeks
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
Deliverables
- Pre-immune serum ~ 5ml each rabbit
- Test serum ~ 5ml each rabbit
- 2 Production sera ~ 10-20 ml each rabbit
Peptide
ProSci-Provided Antigen
Time: 12-13 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen Design
- Peptide synthesis up to 15 aa
- Conjugation to carrier protein
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
- ELISA on 1st bleed
Deliverables
- Pre-immune serum, ~5 ml each rabbit
- Test serum, ~5 ml each rabbit
- 2 Production sera, 10~20 ml each rabbit
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: 13-15 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis up to 15 aa
- Conjugation to carrier protein
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
- ELISA on 1st bleed
1-2 weeks
Purification
- Immuno-affinity purification of 25 ml
serum against peptide
Deliverables
- Pre-immune serum, ~5 ml each rabbit
- Test serum, ~5 ml each rabbit
- 2 Production sera, 10~20 ml each rabbit
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
