Premier
Client-Provided Splenocytes
Time: ~7-12 weeks
2-3 weeks
Phase II: Single-Cell Sorting, Culturing, and Screening
- Splenocyte isolation from 1 rabbit
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
8-13 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
Pro
Client-Provided Antigen
Time: 16-26 weeks
10-15 weeks
Phase I: Immunization
- 3 Rabbits
- 1 Pre-immune serum collection
- 5 Immunizations
- 2 Serum collections
- ELISA at each serum collection
- Splenocyte isolation from 2 rabbits
2-3 weeks
Phase II: Single-cell Sorting, Culturing, and Screening
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
18-23 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
