Monoclonal Antibody Development

Monoclonal Antibody Development Production
Process & Information

Monoclonal antibodies have higher specificity and affinity compared to polyclonal antibodies. ProSci uses hybridoma development for mouse monoclonal antibody development, which is a more established technology compared to recombinant antibody methods.

We have over 25 years of experience developing monoclonal antibodies; working with customers worldwide across a broad range of research areas in academia and industry to take on challenging antibody needs. ProSci antibody experts allow researchers to focus on optimizing other aspects of their research.

Antigen Requirements

Antigen Purity

For monoclonal antibodies, >90% antigen purity is the standard to ensure specificity

Protein Antigen

Quantity: 1mg with no tag at concentrations of 1 mg/ml or higher required for immunization plus 1-2 mg required for screening
Size: at least 10 kDa
Production Method: Use of native protein is preferred over recombinant protein. If supplying a recombinant protein, use an expression system with a similar origin as your protein target (for example, use a mammalian expression system for human proteins) to maximize probability of proper protein conformation and post-translational modifications and increase likelihood of developing an antibody that will recognize the native protein.
Tags: No tags for immunization and large fusion proteins such as GST or MBP must be cleaved to maximize immune response against the target of interest. Tags, however, may be acceptable for screening; contact one of our custom antibody experts to discuss your project today.

Peptide Antigen

Quantity
- 5 mg for us to conjugate, or
- 1 mg conjugated to a carrier protein (KLH preferred), and
- 2-3 mg unconjugated for screening
Antigens in Solution: Antigens should be submitted at a concentration of 1 mg/ml or higher. Please specify the buffer, concentration, total volume, and total amount. Buffers such as PBS, Tris, <4M urea are preferred; SDS and citrate buffers are also acceptable. Please note for services such as ELISA and immuno-affinity purification the antigen needs to be soluble. Please submit an antigen submission form with the information above prior to sending your antigen.

Screening Options

We offer ELISA screening throughout the entire project and can deliver the final positive clones to you!

You can take an active role in your monoclonal antibody development to ensure the right results by screening ELISA-positive supernatants in your lab. Contact our experts to learn more about our screening options. We highly recommend screening positive wells in your lab as this will increase the chances of developing a cell line that produces monoclonal antibodies that work in your target application.

Add-ons

Additonal Clones: If you would like more final clones than the quantity included in a package, we would be happy to assist and can provide additional clones for your project.

Final Clone Isotyping: If you would like us to isotype any additional final clones for your project, we would be happy to assist by providing this service for you.

Additional ELISA Screening: If you need a highly specific monoclonal antibody, we can assist with narrowing down the specificity by screening against multiple samples by ELISA, either positive or negative. Contact our custom antibody experts to discuss your project today.

Our Assurance

Because of the nature of developing antibodies from immunization to final clones, we implement project milestones consisting of clear, actionable go/no-go decision points during the development process. Our experts review the antibody development progress at each milestone to assure antibody success. In addition, if you use a protein, or ProSci selected the peptide immunogen, we guarantee an immune response in Phase I and ELISA positive clones to the immunogen at the end of the project.