Recombinant Single Domain Antibody Production & Development Services
Single Domain Antibodies (sdAbs) from the camelid family are exciting and relatively new tools for researchers. These antibodies, also known as VHH antibodies, are isolated from camelid heavy chain only antibodies and are comprised of only the variable domain. As such, they lack light chains, are smaller and more stable compared to conventional antibodies, yet possess fully functional antigen-binding capability. Due to their size (approximately 15 kDa) and their longer CDR-3 region, a single domain antibody is adept at reaching otherwise inaccessible epitopes that may play a crucial role in the molecular mechanisms of disease. They are highly valuable in fundamental research, diagnostic, and therapeutic applications. ProSci’s recombinant antibody development for single domain antibodies provides a wide variety of custom configurations to accommodate your needs. Using phage display technology and multiple checkpoints throughout development ensures customer satisfaction and quality in developing single domain antibodies.
Phage Display Technology
ProSci utilizes the specialized technology of antibody phage display to generate high-quality single domain antibodies. Our laboratories are equipped with cutting-edge technology and a team of experts skilled in the latest phage display methods. Phage libraries are typically panned in solution in order to develop sdAbs immunoreactive to native, properly folded proteins.
Recombinant Phage Library
Our antibody phage display library construction, panning, and screening methods will meet the demanding and complex needs of your research endeavors. ProSci remains up-to-date with the expertise that discerning researchers have come to trust not only because of our many years of experience but also because of our foundation in research.
Schedule
Single domain antibody development is currently offered in the following phases:
Phase
Week
Procedure
Detailed Procedure
I
12 Weeks
Immunization
- Immunization of 1 llama on standard 12-week protocol
- ELISA testing of serum
II
1 Week
PBMC Isolation
- PBMCs isolated and preserved from blood collected at weeks 8 and 12
III
2-3 Weeks
Library Construction
- sdAb library construction from Phase II PBMCs (customer’s choice of bleed 2 or 3)
IV
4-6 Weeks
Library Panning and Screening
- Up to two rounds of phage library panning against one antigen
- Screening by standard ELISA
V
4-6 Weeks
Single Domain Clone Selection and Sequencing
- Selection and first pass sequence of ELISA-positive binders
- Small-scale production of up to 6 final positive clones
Antigen Requirements
Antigen Purity
- For recombinant single domain antibody development, >90% antigen purity is recommended for specificity and functionality.
Protein Antigen
- Quantity
- 0.5 mg at concentrations of 1 mg/ml or higher for immunization
- 0.5 - 1 mg for screening (quantities varies based on project please inquire)
- Size Ideally greater than 10 kDa
- Production method Using a properly folded native protein is preferred as sdAbs are adept at recognizing conformational epitopes. Therefore, it is ideal for your target protein to come from an expression system similar to the origin of the protein (e.g. use a mammalian expression system for human or other mammalian proteins).
- Tags It is recommended not to have tags for immunization and large fusion proteins such as GST or MBP should be cleaved to minimize nonspecific immune response. Contact us for your project.
Premier Package
Immunization through Library Construction
- Immunization protocol in 1 llama
- PBMC Isolation
- Library Construction
Pro Package
Immunization through Final Clones
- Immunization protocol in 1 llama
- PBMC Isolation
- Library Construction
- Library Panning and Screening
- Single Domain Clone Selection and Sequencing
- Small Scale Single Domain Antibody Production (up to 6 clones)
Add-ons
Additonal Single Domain Antibody If you need additional single domain antibodies than what is included in the package, we offer additional final clones, which include the antibody library in glycerol stock as deliverables.
Additional ELISA Screening If you need additional ELISA screening throughout the project, we offer ELISA screening against additional samples and provide the data as a deliverable.
Custom Screening of Phage Display Library If you need a screening methodology other than ELISA, we offer customizable solutions upon request. Contact one of our custom antibody experts today to discuss your project in detail.
Individual Phases If you would like to start or stop our single domain service at any individual phase of the project, we can accommodate upon request. Contact our experts to discuss your project needs.
Our Assurance
Because of the nature of developing antibodies from immunization to final clones, we implement project milestones consisting of clear, actionable go/no-go decision points during the development process. Our experts review the antibody development progress at each milestone to assure your antibody success.
More Single Domain Antibody Resources
Download the single domain antibodies brochure and listen in on the single domain antibody video from ProSci and Biotechniques. These resources give you insight on what a single domain antibody is and how they are becoming hot tools in the field of infectious disease research.
Hear from two experts in the field of single domain antibody development and production, Andy Lee, Lead Scientist of the Single Domain Antibody Program at ProSci, and David Eling, Director of Business Development at ProSci, on how these unique antibodies are produced and how they are advancing discoveries in research, diagnostic, and therapeutic applications. Listen to the podcast here.
Single Domain Antibody Production
Once antibody development is complete, recombinant single domain antibody production with bacterial expression systems can start. Inquire for more information.
Throughout all phases of development, single domain integrity is ensured with ProSci’s unwavering commitment to customer satisfaction and focus on high quality antibodies. If your research calls for a single domain antibody (VHH antibody), purchase ProSci single domain antibodies with confidence! We have earned the trust of both private and public research sectors, working with a variety of research applications – general research, diagnostic, and pharmaceutical.
