Monoclonal
antibody services
ProSci's Custom Monoclonal Antibody Team are experts in the development and production of antibodies utilizing hybridoma technologies. We have specially-developed techniques to ensure successful hybridoma development with a fusion success rate of over 98%. We guarantee stable cell lines with ELISA-positive clones and careful screening throughout monoclonal antibody development.
Our step-by-step process starts with antigen design and goes through Immunization, ELISA testing of serum, fusion, subcloning, and isotyping of final clones. ProSci's custom process allows flexibility along each step of development allowing for custom options that can be implemented based on our clients' needs.
Contact our specialists today for a free project consultation.
Premier
Client-Provided Splenocytes
Time: ~7-12 weeks
2-3 weeks
Phase II: Single-Cell Sorting, Culturing, and Screening
- Splenocyte isolation from 1 rabbit
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
8-13 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
Pro
Client-Provided Antigen
Time: 16-26 weeks
10-15 weeks
Phase I: Immunization
- 3 Rabbits
- 1 Pre-immune serum collection
- 5 Immunizations
- 2 Serum collections
- ELISA at each serum collection
- Splenocyte isolation from 2 rabbits
2-3 weeks
Phase II: Single-cell Sorting, Culturing, and Screening
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
18-23 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
Premier
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 3 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 1 ELISA-positive clone
Pro
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
Plus
ProSci-Provided Antigen
Time: ~22-30 weeks
4-5 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 25 aa at >70% purity
- Conjugation to carrier protein
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
Package Add-ons
- Additional ELISA Screening
- Additional Final Clones
- Affinity Measurement
- Antibody Sequencing
- Endotoxin testing and removal
- Final Clone Isotyping
- Flow Cytometry Screening
- In vitro production
Our Process
