Polyclonal
antibody services
ProSci's polyclonal antibody services are available in a wide variety of custom configurations and standard packages to fit your research needs. Custom immunogen design, experience with multiple adjuvants, and multiple species to choose for immunizations ensure your custom project will be a success. Our antibody experts and professional project managers focus on saving you time, and costs, and optimizing all aspects of your project to accelerate you into your next discovery.
The packages below can be customized or provided as described. Please contact us for a consultation on your project today!
Serum
Client-Provided Antigen
Time: ~13 weeks
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
Peptide
ProSci-Provided Antigen
Time: ~16-17 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: ~17-19 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
1-2 weeks
Purification
- Immuno-affinity purification of 25 ml
serum against peptide
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
IgY
Client-Provided Antigen
Time: ~9-10 weeks
~11-12 weeks
Immunization Protocol
- 2 Chickens
- Pre-immune IgY purification
- 4 Immunizations per chicken
- 3 Test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
Deliverables
- 5 mg pre-immune IgY
- 3 test serum samples, ~1 ml each chicken
- ~1000 mg immune IgY
Peptide
ProSci Provided Antigen
Time: ~11-13 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
11-12 weeks
Immunization Protocol
- 2 Chickens
- 4 Immunizations per chicken
- 3 test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
Deliverables
- 3 test sera samples, ~1 ml each chicken
- ~1000 mg immune IgY
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Yolk
Purification
Time: ~12-15 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
11-12 weeks
Immunization Protocol
- 2 chickens
- 4 immunizations per chicken
- 3 test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
1-2 weeks
Immuno-Affinity Purification
- Immuno-affinity purification of 25 mg IgY against peptide
Deliverables
- 3 Test sera samples, ~1 ml each chicken
- ~1000 mg immune IgY
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
Serum
Client-Provided Antigen
Time: 9 weeks
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
Deliverables
- Pre-immune serum ~ 5ml each rabbit
- Test serum ~ 5ml each rabbit
- 2 Production sera ~ 10-20 ml each rabbit
Peptide
ProSci-Provided Antigen
Time: 12-13 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen Design
- Peptide synthesis up to 15 aa
- Conjugation to carrier protein
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
- ELISA on 1st bleed
Deliverables
- Pre-immune serum, ~5 ml each rabbit
- Test serum, ~5 ml each rabbit
- 2 Production sera, 10~20 ml each rabbit
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: 13-15 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis up to 15 aa
- Conjugation to carrier protein
9 weeks
Immunization Protocol
- 2 Rabbits
- 1 Pre-immune serum collection
- 4 Immunizations per rabbit
- 3 Serum collections per rabbit
- ELISA on 1st bleed
1-2 weeks
Purification
- Immuno-affinity purification of 25 ml
serum against peptide
Deliverables
- Pre-immune serum, ~5 ml each rabbit
- Test serum, ~5 ml each rabbit
- 2 Production sera, 10~20 ml each rabbit
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
Serum
Client-Provided Antigen
Time: ~8 weeks
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- Production serum collection, ~0.5ml each mouse
Peptide
ProSci-Provided Antigen
Time: ~11-12 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- Production serum collection, ~0.5ml each mouse
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: ~12-14 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
1-2 weeks
Purification
- Immuno-affinity purification of serum against peptide
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
Serum
Client-Provided Antigen
Time: ~13-14 weeks
13-14 weeks
Immunization Protocol
- 1 Llama or alpaca
- 1 Pre-immune serum collection
- 4 Immunizations per llama or alpaca
- 3 Production serum collections per llama or alpaca
Deliverables
- Pre-immune serum, ~25 ml each llama/alpaca
- 3 Production serum collections, ~150-250ml each llama/alpaca
Package Add-ons
- Purify Additional Sera - Available for all Packages
- Modification-Specific Antibodies (e.g. phospho)
- Peptide Cocktail
- Additional ELISAs
- Antibody Labeling
Not seeking the service you need? Just ask!
Our Process
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