Custom Recombinant Services
Antibody Development and Production
ProSci's Custom Recombinant Antibody Team are experts in the development and production of antibodies utilizing phage display or B cell cloning.
Our step-by-step process starts with antigen design and goes through immunization, single-cell sorting, culturing, and screening, and completes with cloning, small-scale expression, and delivery of a research-ready antibody. ProSci's custom process allows flexibility along each step of development allowing for custom options that can be implemented based on our client's needs.
Contact our specialists today for a free project consultation.
Premier
Client-Provided Splenocytes
Time: ~7-12 weeks
2-3 weeks
Phase II: Single-Cell Sorting, Culturing, and Screening
- Splenocyte isolation from 1 rabbit
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
8-13 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
Pro
Client-Provided Antigen
Time: 16-26 weeks
10-15 weeks
Phase I: Immunization
- 3 Rabbits
- 1 Pre-immune serum collection
- 5 Immunizations
- 2 Serum collections
- ELISA at each serum collection
- Splenocyte isolation from 2 rabbits
2-3 weeks
Phase II: Single-cell Sorting, Culturing, and Screening
- Sorting and culturing antibody secreting B-cells
- ELISA screening
4-8 weeks
Phase III: Antibody Cloning and Small-Scale Expression
- Cloning variable regions from B-cells
- Transient expression of recombinant antibodies
18-23 weeks
Phase IV: Sequencing, Expression, and Purification
- Sequencing heavy and light chains
- Expression and purification of up to 5 antibodies
Deliverables
- ELISA-positive B-cell supernatants, up to 96
- ELISA-positive recombinant supernatants, up to 10
- 100ug purified antibody from up to 5 clones
- DNA and sequences of up to 5 final clones
Premier
Immunization through Library Construction
Time: 11-17 weeks
8-12 weeks
Phase I: Immunization
- 1 Llama or alpaca
- 1 Pre-immune serum collection
- 4 Immunizations
- 3 Test serum collections
- ELISA on each bleed
1 week
Phase II: PBMC Isolation
- PBMC isolation weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
- sdAb library construction from Phase II PBMCs
Deliverables
- sdAb library as glycerol stock of phage plasmid-containing bacteria
Pro
Immunization through Final Clones
Time: 17-25 weeks
8-12 weeks
Phase I: Immunization
- 1 Llama or alpaca
- 1 Pre-immune serum collection
- 4 Immunizations
- 3 Test serum collections
- ELISA on each bleed
1 week
Phase II: PBMC Isolation
- PBMC isolation at weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
- sdAb library construction from Phase II PBMCs
2-3 weeks
Phase IV: Library Panning and Screening
- 2 rounds of panning
- ELISA screening
4-6 weeks
Phase V: Single Domain Clone Selection and Sequencing
- Selection and sequencing of binders
- Small-scale antibody production
Deliverables
- Amino acid sequences of binders
- Purified antibody from up to 6 final clones
- Confirmed DNA sequences and glycerol stocks of final clones
Package Add-ons
- Additional ELISA
- Additional Final Clones
- Affinity Measurement
- Antigen Production
- Custom Panning of Phage Display Library**
- Endotoxin Testing and Removal
- Flow Cytometry Screening
- Protein A/G Purification
- Recombinant Antibody Production
- WB Screening*
*rabbit monoclonal only ** single domain only
Our Process
