Monoclonal
antibody services
ProSci's Custom Monoclonal Antibody Team are experts in the development and production of antibodies utilizing hybridoma technologies. We have specially-developed techniques to ensure successful hybridoma development with a fusion success rate of over 98%. We guarantee stable cell lines with ELISA-positive clones and careful screening throughout monoclonal antibody development.
Our step-by-step process starts with antigen design and goes through Immunization, ELISA testing of serum, fusion, subcloning, and isotyping of final clones. ProSci's custom process allows flexibility along each step of development allowing for custom options that can be implemented based on our clients' needs.
Contact our specialists today for a free project consultation.
Premier
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 3 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 1 ELISA-positive clone
Pro
Client-Provided Antigen
Time: ~18-25 weeks
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
Plus
ProSci-Provided Antigen
Time: ~22-30 weeks
4-5 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 25 aa at >90% purity
- Conjugation to carrier protein
12-16 weeks
Phase I: Immunization
- 5 BALB/c mice
- 1 Pre-immune serum collection
- 5 Immunizations
- 4 Serum collections
- ELISA testing of serum
2-3 weeks
Phase II: Fusion
- Fusion of 1 mouse spleen
- ELISA screening
4-6 weeks
Phase III: Subcloning
- 2 subcloning steps
- Final clone isotyping
Deliverables
- ELISA data
- Supernatant for testing (optional)
- 3 ELISA-positive clones
Package Add-ons
- Additional ELISA Screening
- Additional Final Clones
- Affinity Measurement
- Antibody Sequencing
- Endotoxin testing and removal
- Final Clone Isotyping
- Flow Cytometry Screening
- In vitro production
Our Process
