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Custom Recombinant Services
Antibody Development and Production

ProSci's Custom Recombinant Antibody Team are experts in the development and production of antibodies utilizing phage display or B cell cloning.

Our step-by-step process starts with antigen design and goes through Immunization, single-cell sorting, culturing, and screening, and completes with cloning, small-scale expression, and delivery of a research-ready antibody. ProSci's custom process allows flexibility along each step of development allowing for custom options that can be implemented based on our client's needs.

Contact our specialists today for a free project consultation.

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Single Domain Antibody Services

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Premier

Client-Provided Splenocytes

Time: ~7-12 weeks

2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
  • Plasma cell isolation from 1 rabbit
  • RNA isolation, cDNA synthesis
  • Amplification of heavy and light chains
  • Cloning into expression vector
  • ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
  • Deconvolution of ELISA-positive clones (customer-selected)
  • Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
  • Supernatant from ELISA-positive wells
  • Purified antibody from up to 3 clones
  • DNA and sequences of 2 final clones
  • ELISA report

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Pro

Client-Provided Antigen

Time: 16-26 weeks

10-15 weeks
Phase I: Immunization
  • 3 Rabbits
  • 1 Pre-immune serum collection
  • 5 Immunizations
  • 2 Serum collections
  • ELISA at each serum collection
2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
  • Plasma cell isolation from 1 rabbit
  • RNA isolation, cDNA synthesis
  • Amplification of heavy and light chains
  • Cloning into an expression vector
  • ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
  • Deconvolution of ELISA-positive clones (customer-selected)
  • Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
  • Supernatant from ELISA-positive wells
  • Purified antibody from up to 3 clones
  • DNA and sequences of 2 final clones
  • ELISA report

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Premier

Immunization through Library Construction

Time: 11-17 weeks

8-12 weeks
Phase I: Immunization
  • 1 Llama or alpaca
  • 1 Pre-immune serum collection
  • 4 Immunizations
  • 3 Test serum collections
  • ELISA on each bleed
1 week
Phase II: PBMC Isolation
  • PBMC isolation weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
  • sdAb library construction from Phase II PBMCs
Deliverables
  • sdAb library as glycerol stock of phage plasmid-containing bacteria

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Pro

Immunization through Final Clones

Time: 17-25 weeks
8-12 weeks
Phase I: Immunization
  • 1 Llama or alpaca
  • 1 Pre-immune serum collection
  • 4 Immunizations
  • 3 Test serum collections
  • ELISA on each bleed
1 week
Phase II: PBMC Isolation
  • PBMC isolation at weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
  • sdAb library construction from Phase II PBMCs
2-3 weeks
Phase IV: Library Panning and Screening
  • 2 rounds of panning
  • ELISA screening
4-6 weeks
Phase V: Single Domain Clone Selection and Sequencing
  • Selection and sequencing of binders
  • Small-scale antibody production
Deliverables
  • Amino acid sequences of binders
  • Purified antibody from up to 6 final clones
  • Confirmed DNA sequences and glycerol stocks of final clones

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Package Add-ons
  • Additional Final Clones
  • Additional ELISA
  • Antibody Production**
  • Custom Panning of Phage Display Library**
  • Endotoxin Testing and Removal
  • Affinity Measurement
  • Flow Cytometry Screening

** single domain only

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Our Process

ProSci custom antibody service process

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Need Help?
Learn more in our resources:
  • Rabbit Monoclonal Information
  • Antibody Development Guide
  • Single Domain Information

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P +1 (858) 513-2638
F +1 (858) 513-2692

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