Custom Recombinant
Antibody Production & Development Services
ProSci offers custom recombinant antibody development services for rabbit monoclonal antibodies and llama single domain antibodies. We understand researchers have a wide array of needs and levels of involvement in developing recombinant antibodies depending on your target. With our flexible services, broad range of antibody development expertise, and enhanced capabilities, we meet your antibody success needs and provide custom recombinant antibody solutions.
Premier
Immunization through Library Construction
Time: ~11-16 weeks
8-12 weeks
Phase I: Immunization
- 1 Llama
- 1 Pre-immune serum collection
- 4 Immunizations
- 3 Test serum collections
- ELISAs on each bleed
8-12 weeks
Phase II: PBMC Isolation
- PBMC isolation from week 8 and 12 bleeds
1 week
Phase III: cDNA Library Construction
- sdAb library as glycerol stock of phage plasmid-containing bacteria
Deliverables
- sdAb library as glycerol stock of phage plasmid-containing bacteria (optional)
Pro
Immunization through Final Clones
Time: 19-28 weeks
8-12 weeks
Phase I: Immunization
- 1 Llama
- 1 Pre-immune serum collection
- 4 Immunizations
- 3 Test serum collections
- ELISAs on each bleed
1 week
Phase II: PBMC Isolation
- PBMC isolation from serum collected at weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
- sdAb library construction from Phase II PBMCs
2-3 weeks
Phase IV: Library Panning and Screening
- 2 rounds of panning
- ELISA screening
4-6 weeks
Phase V: Single Domain Clone Selection and Sequencing
- Selection and sequencing of binders
- Small-scale antibody production
Deliverables
- Amino acid sequences of binders
- Purified antibody from up to 6 final clones
- Confirmed DNA sequences and glycerol stocks of final clones
Premier
Client-Provided Splenocytes
Time: ~7-12 weeks
2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
- Plasma cell isolation from 1 rabbit
- RNA isolation, cDNA synthesis
- Amplification of heavy and light chains
- Cloning into expression vector
- ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
- Deconvolution of ELISA-positive clones (customer-selected)
- Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
- Purified antibody from up to 3 clones
- DNA and sequences of 2 final clones
- ELISA report
Pro
Client-Provided Antigen
Time: 10-15 weeks
10-15 weeks
Phase I: Immunization
- 3 Rabbits
- 1 Pre-immune serum collection
- 5 Immunizations
- 2 Serum collections
- ELISA at each serum collection
2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
- Plasma cell isolation from 1 rabbit
- RNA isolation, cDNA synthesis
- Amplification of heavy and light chains
- Cloning into an expression vector
- ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
- Deconvolution of ELISA-positive clones (customer-selected)
- Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
- Supernatant from ELISA-positive wells
- Purified antibody from up to 3 clones
- DNA and sequences of 2 final clones
- ELISA report
Package Add-ons
- Additional Final Clones
- Additional ELISA
- Protein G Purification*
- Single Domain Antibody Production**
- Custom Panning of Phage Display Library**
* rabbit monoclonal only ** single domain only
Our Process
Get Started
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Need Help?
Learn more in our resources:
Rabbit Monoclonal InformationAntibody Development Guide
Single Domain Information
