Llama (Recombinant)

Premier

Immunization through Library Construction

Time: ~11-16 weeks

8-12 weeks
Phase I: Immunization
  • 1 Llama
  • 1 Pre-immune serum collection
  • 4 Immunizations
  • 3 Test serum collections
  • ELISAs on each bleed
8-12 weeks
Phase II: PBMC Isolation
  • PBMC isolation from week 8 and 12 bleeds
1 week
Phase III: cDNA Library Construction
  • sdAb library as glycerol stock of phage plasmid-containing bacteria
Deliverables
  • sdAb library as glycerol stock of phage plasmid-containing bacteria (optional)

Pro

Immunization through Final Clones

Time: 19-28 weeks

8-12 weeks
Phase I: Immunization
  • 1 Llama
  • 1 Pre-immune serum collection
  • 4 Immunizations
  • 3 Test serum collections
  • ELISAs on each bleed
1 week
Phase II: PBMC Isolation
  • PBMC isolation from serum collected at weeks 8 and 12
2-3 weeks
Phase III: cDNA Library Construction
  • sdAb library construction from Phase II PBMCs
2-3 weeks
Phase IV: Library Panning and Screening
  • 2 rounds of panning
  • ELISA screening
4-6 weeks
Phase V: Single Domain Clone Selection and Sequencing
  • Selection and sequencing of binders
  • Small-scale antibody production
Deliverables
  • Amino acid sequences of binders
  • Purified antibody from up to 6 final clones
  • Confirmed DNA sequences and glycerol stocks of final clones