Mouse

Serum

Client-Provided Antigen

Time: ~8 weeks

8 weeks
Immunization Protocol
  • 5 Mice
  • 1 Pre-immune serum collection
  • 4 Immunizations per mouse
  • 1 Test serum collection
  • 1 Production serum collection
Deliverables
  • Pre-immune serum, ~0.05 ml each mouse
  • Test serum, ~0.05 ml each mouse
  • Production serum collection, ~0.5ml each mouse

Peptide

ProSci-Provided Antigen

Time: ~11-12 weeks

3-4 weeks
Antigen Design & Synthesis
  • Antigen design
  • Peptide synthesis of up to 15 aa
  • Conjugation to carrier protein
8 weeks
Immunization Protocol
  • 5 Mice
  • 1 Pre-immune serum collection
  • 4 Immunizations per mouse
  • 1 Test serum collection
  • 1 Production serum collection
Deliverables
  • Pre-immune serum, ~0.05 ml each mouse
  • Test serum, ~0.05 ml each mouse
  • Production serum collection, ~0.5ml each mouse
  • 5 mg peptide
  • ELISA report

Purified

ProSci-Provided Antigen + Serum
Purification

Time: ~12-14 weeks

3-4 weeks
Antigen Design & Synthesis
  • Antigen design
  • Peptide synthesis of up to 15 aa
  • Conjugation to carrier protein
8 weeks
Immunization Protocol
  • 5 Mice
  • 1 Pre-immune serum collection
  • 4 Immunizations per mouse
  • 1 Test serum collection
  • 1 Production serum collection
1-2 weeks
Purification
  • Immuno-affinity purification of serum against peptide
Deliverables
  • Pre-immune serum, ~0.05 ml each mouse
  • Test serum, ~0.05 ml each mouse
  • 5 mg peptide
  • ELISA report
  • Purified antibody
  • Immuno-affinity column
  • Purification report