Premier
Client-Provided Splenocytes
Time: ~7-12 weeks
2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
- Plasma cell isolation from 1 rabbit
- RNA isolation, cDNA synthesis
- Amplification of heavy and light chains
- Cloning into expression vector
- ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
- Deconvolution of ELISA-positive clones (customer-selected)
- Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
- Supernatant from ELISA-positive wells
- Purified antibody from up to 3 clones
- DNA and sequences of 2 final clones
- ELISA report
Pro
Client-Provided Antigen
Time: 16-26 weeks
10-15 weeks
Phase I: Immunization
- 3 Rabbits
- 1 Pre-immune serum collection
- 5 Immunizations
- 2 Serum collections
- ELISA at each serum collection
2-3 weeks
Phase II: Plasma Cell Isolation and Sorting
- Plasma cell isolation from 1 rabbit
- RNA isolation, cDNA synthesis
- Amplification of heavy and light chains
- Cloning into an expression vector
- ELISA screening
4-8 weeks
Phase III: Gene Amplification, Cloning, Expression, and Screening
- Deconvolution of ELISA-positive clones (customer-selected)
- Small-scale antibody production of up to 3 ELISA-positive clones
Deliverables
- Supernatant from ELISA-positive wells
- Purified antibody from up to 3 clones
- DNA and sequences of 2 final clones
- ELISA report
